Controlled cell growth using holographic optical traps

Authors

  • Godofredo Bautista ⋅ PH National Institute of Physics, University of the Philippines Diliman
  • Reniel Cabral ⋅ PH National Institute of Physics, University of the Philippines Diliman
  • Mary Grace Bato ⋅ PH National Institute of Physics, University of the Philippines Diliman
  • Vincent Ricardo Daria ⋅ AU National Institute of Physics, University of the Philippines Diliman and Physics Department, Australian National University

Abstract

We demonstrate controlled growth of yeast cells using holographic optical traps generated by encoding a computer-designed hologram on a phase-only spatial light modulator. A Ti:S femtosecond laser operating at a wavelength of 790 nm under continuous-wave (CW) mode is used as the trapping source. We show simultaneous trapping of three growing yeast cells that are initially beside each other under a controlled environment. This protocol benchmarks a tool for initially studying the growth relationships between confined cells in mixed cultures.

[Reprinted from: G Bautista, R Cabral, MG Bato, and V Daria, "Controlled cell growth using holographic optical traps," in Proceedings of the 26th Samahang Pisika ng Pilipinas Physics Congress (Baguio City, 2008), SPP-2008-4D-03.]

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Published

2008-10-01

Issue

Vol. 2 No. 1: October 2008

Section

Brief Communications