Fluorescence suppression and surface-enhanced Raman scattering (SERS) in rhodamine B dye using silver nanowires as SERS substrates

  • Horace Andrew Fernandez Husay National Institute of Physics, University of the Philippines Diliman
  • Jeremias Ibus-Armonia National Institute of Physics, University of the Philippines Diliman
  • Laureen Ida Monzon Ballesteros Materials Science and Engineering Program, University of the Philippines Diliman
  • Debinya Banisal Buenafe Materials Science and Engineering Program, University of the Philippines Diliman
  • Ferdinand Tamayo Cayabyab, Jr National Institute of Physics, University of the Philippines Diliman
  • Nathaniel T De Guzman Department of Mining, Metallurgical, and Materials Engineering, University of the Philippines Diliman
  • Mary Donnabelle L Balela Department of Mining, Metallurgical, and Materials Engineering, University of the Philippines Diliman
  • Elmer Surat Estacio National Institute of Physics, University of the Philippines Diliman
  • Arnel Angud Salvador National Institute of Physics, University of the Philippines Diliman
  • Armando Soriano Somintac National Institute of Physics, University of the Philippines Diliman

Abstract

We investigated the fluorescence suppression and surface-enhanced Raman scattering (SERS) in rhodamine B dye using silver nanowires (Ag NWs) as SERS substrates. The Ag NWs were prepared by electroless deposition and subsequently suspended in ethanol. The NWs were deposited on paper and used to detect rhodamine B (RhB) in water to a concentration of 5 µM. Fluorescence and Raman measurements were performed using a micro-Raman spectroscopy setup with a 532 nm excitation. Optical microscope images showed the presence of fluorescent regions where only a few NWs were observed and dark regions where a dense amount of Ag NWs were located. The fluorescence emission centered at ~581 nm was attributed to the RhB dye. A ~75% decrease in the fluorescence emission was observed at the SERS hotspots with Ag NWs. Raman measurements performed at the Ag NW-filled region confirmed that the vibrational modes of RhB were enhanced by a factor of 104. This study demonstrates the feasibility of using Ag NWs to suppress fluorescence in biological or inorganic samples for Raman microscopy.

Published
2018-06-01
How to Cite
[1]
H. A. Husay, J. Ibus-Armonia, L. I. Ballesteros, D. Buenafe, F. Cayabyab, N. De Guzman, M. D. Balela, E. Estacio, A. Salvador, and A. Somintac. Fluorescence suppression and surface-enhanced Raman scattering (SERS) in rhodamine B dye using silver nanowires as SERS substrates, Proceedings of the Samahang Pisika ng Pilipinas 36, SPP-2018-PC-52 (2018). URL: https://paperview.spp-online.org/proceedings/article/view/SPP-2018-PC-52.
Section
Poster Session C (Mathematical Physics, Optics, and Interdisciplinary Topics)